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Product Name
FBF1 antibody
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Description
FBF1 Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in mouse liver tissue, COLO 320 cells, L02 cells. Positive IHC detected in human colon cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 130 kDa,90 kDa,70 kDa
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Tested applications
ELISA, IHC, WB, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
Alb antibody; albatross antibody; Fas (TNFRSF6) binding factor 1 antibody; FBF 1 antibody; FBF1 antibody; FBF1 Ens antibody; FLJ00103 antibody; FLJ13996 antibody; HCG1989313 antibody; isoform CRA_b antibody; KIAA1863 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FBF1 recombinant protein (Accession Number: BC023549). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:110535(FBF1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:110535(FBF1 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-FBF1 (IP:Catalog No:110535, 4ug; Detection:Catalog No:110535 1:300) with mouse liver tissue lysate 4000ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:110535(FBF1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
FBF1, also named as ALB and KIAA1863, is keratin-binding protein required for epithelial cell polarization. It is involved in apical junction complex (AJC) assembly via its interaction with PARD3. FBF1 is required for ciliogenesis. FBF1 has six isoforms with MW 125-127 kDa, 99 kDa, 69 kDa and 55 kDa. This antibody is induced by N-terminal (20-347aa) of FBF1. It recognizes all the six isoforms.
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References
- Wei Q, Xu Q, Zhang Y. Transition fibre protein FBF1 is required for the ciliary entry of assembled intraflagellar transport complexes. Nature communications. 4:2750. 2013.
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