Cart 2

FAS/CD95 antibody

Cat.#: 110527

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    FAS/CD95 antibody
  • Documents
  • Description
    FAS/CD95 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, Jurkat cells. Positive IHC detected in human prostate cancer tissue, human colon cancer tissue. Positive FC detected in Jurkat cells. Observed molecular weight by Western-blot: 38-45 kDa
  • Tested applications
    ELISA, WB, IHC, IP, FC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    ALPS1A antibody; APO 1 antibody; Apo 1 antigen antibody; APT1 antibody; CD95 antibody; FAS antibody; FAS1 antibody; FASLG receptor antibody; FASTM antibody; TNFRSF6 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of FAS/CD95 recombinant protein (Accession Number: NM_000043). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110527(FAS antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110527(FAS antibody) at dilution of 1:1000

    IP Result of anti-FAS (IP:Catalog No:110527, 3ug; Detection:Catalog No:110527 1:500) with HeLa cells lysate 3000ug.

    IP Result of anti-FAS (IP:Catalog No:110527, 3ug; Detection:Catalog No:110527 1:500) with HeLa cells lysate 3000ug.

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:110527(FAS antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:110527(FAS antibody) at dilution of 1:100 (under 10x lens)

    1X10^6 Jurkat cells were stained with 0.2ug FAS antibody (Catalog No:110527, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 Jurkat cells were stained with 0.2ug FAS antibody (Catalog No:110527, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    Fas (CD95/APO-1) is a transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. It can mediates apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The molecular mass of native Fas is 38 kDa, the high molecular weight form (40-55 kDa) of Fas is due to glycosylation.
  • References
    • Chen W, Hou J, Yin Y. alpha-Bisabolol induces dose- and time-dependent apoptosis in HepG2 cells via a Fas- and mitochondrial-related pathway, involves p53 and NFkappaB. Biochemical pharmacology. 80(2):247-54. 2010.
    • Yin Y, Chen W, Tang C. NF-κB, JNK and p53 pathways are involved in tubeimoside-1-induced apoptosis in HepG2 cells with oxidative stress and G₂/M cell cycle arrest. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 49(12):3046-54. 2011.
    • Bucciantini M, Nosi D, Forzan M. Toxic effects of amyloid fibrils on cell membranes: the importance of ganglioside GM1. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 26(2):818-31. 2012.
    • Yang X, Xu Y, Guo F. Hydroxysteroid sulfotransferase SULT2B1b promotes hepatocellular carcinoma cells proliferation in vitro and in vivo. PloS one. 8(4):e60853. 2013.
    • Wang Q, Dong Z, Liu X. Programmed cell death-4 deficiency prevents diet-induced obesity, adipose tissue inflammation, and insulin resistance. Diabetes. 62(12):4132-43. 2013.
    • Liang Z, Guo YT, Yi YJ, Wang RC, Hu QL, Xiong XY. Ganoderma lucidum polysaccharides target a Fas/caspase dependent pathway to induce apoptosis in human colon cancer cells. Asian Pacific journal of cancer prevention : APJCP. 15(9):3981-6. 2014.
    • Zhang M, Yang J, Shu J. Cytotoxicity induced by nanobacteria and nanohydroxyapatites in human choriocarcinoma cells. Nanoscale research letters. 9(1):616. 2014.
    • Huang Y, Du Y, Zhang X. Down-regulated expression of Annexin A7 induces apoptosis in mouse hepatocarcinoma cell line by the intrinsic mitochondrial pathway. Biomedicine & pharmacotherapy = Biomédecine & pharmacothérapie. 70:146-50. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"