-
Product Name
FAF2 antibody
- Documents
-
Description
FAF2 Rabbit Polyclonal antibody. Positive IF detected in PC-3 cells. Positive IHC detected in human testis tissue. Positive WB detected in mouse brain tissue, HeLa cells, HepG2 cells, human brain tissue, human heart tissue, human pancreas tissue, mouse heart tissue, mouse lung tissue, mouse pancreas tissue, mouse testis tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 53 kDa
-
Tested applications
ELISA, WB, IHC, IP, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
ETEA antibody; FAF2 antibody; FAS assOCiated factor 2 antibody; KIAA0887 antibody; Protein ETEA antibody; UBXD8 antibody; UBXN3B antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of FAF2 recombinant protein (Accession Number: NM_014613). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110452(FAF2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human testis using Catalog No:110452(FAF2 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-FAF2 (IP:Catalog No:110452, 3ug; Detection:Catalog No:110452 1:1000) with mouse brain tissue lysate 5000ug.
Immunofluorescent analysis of PC-3 cells using Catalog No:110452(FAF2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
Protein ETEA (FAF2, or UBXD8) is a homolog to Fas-associated factor 1 (FAF1), which is involved in Fas-mediated apoptosis. ETEA protein directly interacts with and negatively regulates neurofibromin. ETEA contains both UBA and UBX domains and overexpression of ETEA downregulates neurofibromin in human cells. It may play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. ETEA is highly expressed in peripheral blood of patients with atopic dermatitis (AD) compared to normal individuals, and may regulate the resistance to apoptosis that is observed in T cells and eosinophils of AD patients.
-
References
- Xu Y, Liu Y, Lee JG, Ye Y. A ubiquitin-like domain recruits an oligomeric chaperone to a retrotranslocation complex in endoplasmic reticulum-associated degradation. The Journal of biological chemistry. 288(25):18068-76. 2013.
- Lee JG, Kim W, Gygi S, Ye Y. Characterization of the deubiquitinating activity of USP19 and its role in endoplasmic reticulum-associated degradation. The Journal of biological chemistry. 289(6):3510-7. 2014.
- Liu Y, Soetandyo N, Lee JG. USP13 antagonizes gp78 to maintain functionality of a chaperone in ER-associated degradation. eLife. 3:e01369. 2014.
- Zhang T, Xu Y, Liu Y, Ye Y. gp78 functions downstream of Hrd1 to promote degradation of misfolded proteins of the endoplasmic reticulum. Molecular biology of the cell. 26(24):4438-50. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"