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Product Name
EYA3 antibody
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Description
EYA3 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, L02 cells. Positive IP detected in L02 cells. Positive IF detected in Hela cells. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 63-66 kDa
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Tested applications
ELISA, IF, IHC, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp686C132 antibody; EYA3 antibody; Eyes absent homolog 3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EYA3 recombinant protein (Accession Number: NM_172098). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunofluorescent analysis of Hela cells, using EYA3 antibody Catalog No:110422 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110422(EYA3 Antibody) at dilution of 1:600
IP Result of anti-EYA3 (IP:Catalog No:110422, 4ug; Detection:Catalog No:110422 1:1000) with L02 cells lysate 1800ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:110422(EYA3 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:110422(EYA3 Antibody) at dilution of 1:50 (under 40x lens)
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Background
EYA3(Eyes absent homolog 3) belongs to the HAD-like hydrolase superfamily.Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis.EYA3, like EYA1, is expressed in the ninth week of human development and may also underlie developmental defects(PMID:9020840).It has 2 isoforms produced by alternative splicing.This antibody is specific to EYA3.
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References
- Pandey RN, Wang TS, Tadjuidje E, McDonald MG, Rettie AE, Hegde RS. Structure-activity relationships of benzbromarone metabolites and derivatives as EYA inhibitory anti-angiogenic agents. PloS one. 8(12):e84582. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"