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Product Name
EXOSC9 antibody
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Description
EXOSC9 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, HEK-293 cells, MCF-7 cells. Positive IHC detected in human prostate hyperplasia tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 65 kDa
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human; other species not tested.
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Alternative names
Autoantigen PM/Scl 1 antibody; EXOSC9 antibody; exosome component 9 antibody; PM/Scl 75 antibody; PMSCL1 antibody; RRP45 antibody; Rrp45p antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EXOSC9 recombinant protein (Accession Number: NM_005033). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:110415(EXOSC9 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human prostate hyperplasia slide using Catalog No:110415(EXOSC9 Antibody) at dilution of 1:50
Immunofluorescent analysis of HeLa cells using Catalog No:110415(EXOSC9 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
EXOSC9, also named as PMSCL1 and PM/Scl-75, is a key subunit of the exosome complex. EXOSC9 maintains self-renewal in a cell-autonomous manner. EXOSC9 has 4 isoforms with MW 49 kDa, 51 kDa, 39 kDa and 41 kDa. It’s polymyositis/scleroderma auto-antigen 75 kDa. The MW of EXOSC9 is migrated to 60-75 kDa for modification in WB detection.
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