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Product Name
EXOSC10 antibody
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Description
EXOSC10 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, mouse spleen tissue. Positive IF detected in HeLa cells, NIH/3T3 cells. Positive IHC detected in human spleen tissue, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human testis tissue. Observed molecular weight by Western-blot: 100 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
Autoantigen PM/Scl 2 antibody; EXOSC10 antibody; exosome component 10 antibody; PM Scl antibody; PM/Scl 100 antibody; PMSCL antibody; PMSCL2 antibody; RRP6 antibody; Rrp6p antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EXOSC10 recombinant protein (Accession Number: NM_001001998). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110507(EXOSC10 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human spleen using Catalog No:110507(EXOSC10 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human spleen using Catalog No:110507(EXOSC10 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-EXOSC10 (IP:Catalog No:110507, 4ug; Detection:Catalog No:110507 1:700) with HeLa cells lysate 1200ug.
Immunofluorescent analysis of HeLa cells using Catalog No:110507(EXOSC10 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear/nucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.
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