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Product Name
ESR2 antibody
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Description
ESR2 Rabbit Polyclonal antibody. Positive WB detected in NIH/3T3 cells, human brain tissue, mouse brain tissue, mouse testis tissue, PC-3 cells, SH-SY5Y cells. Positive IHC detected in human cervix tissue, human ovary tumor tissue. Observed molecular weight by Western-blot: 50 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ER BETA antibody; Erb antibody; ESR BETA antibody; ESR2 antibody; ESRB antibody; ESTRB antibody; estrogen receptor 2 (ER beta) antibody; Estrogen receptor beta antibody; NR3A2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ESR2 recombinant protein (Accession Number: XM_047431078). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human cervix using Catalog No:110474(ESR2 antibody) at dilution of 1:50 (under 10x lens)
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:110474(ESR2 Antibody) at dilution of 1:2000
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Background
Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual
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References
- Zheng N, Liu L, Liu W. ERβ up-regulation was involved in silibinin-induced growth inhibition of human breast cancer MCF-7 cells. Archives of biochemistry and biophysics. 591:141-9. 2016.
- He YQ, Sheng JQ, Ling XL. Estradiol regulates miR-135b and mismatch repair gene expressions via estrogen receptor-β in colorectal cells. Experimental & molecular medicine. 44(12):723-32. 2012.
- Tang H, Liao Y, Xu L. Estrogen and insulin-like growth factor 1 synergistically promote the development of lung adenocarcinoma in mice. International journal of cancer. 133(10):2473-82. 2013.
- Zhang C, Peng J, Wu S. Dioscin promotes osteoblastic proliferation and differentiation via Lrp5 and ER pathway in mouse and human osteoblast-like cell lines. Journal of biomedical science. 21:30. 2014.
- Fu C, Xu D, Wang CY. Alpha-Lipoic Acid Promotes Osteoblastic Formation in H2O2 -Treated MC3T3-E1 Cells and Prevents Bone Loss in Ovariectomized Rats. Journal of cellular physiology. 230(9):2184-201. 2015.
- Ploskonka SD, Eaton JL, Carr MS, Schmidt JV, Cushing BS. Developmental expression of estrogen receptor beta in the brain of prairie voles (Microtus ochrogaster). Developmental psychobiology. 58(2):223-30. 2016.
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