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Product Name
ERP29 antibody
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Description
ERP29 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human liver tissue, human pancreas tissue. Positive WB detected in mouse liver tissue, HEK-293 cells, mouse kidney tissue, mouse lung tissue. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 29 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
C12orf8 antibody; ERp28 antibody; ERP29 antibody; ERp31 antibody; PDI DB antibody; PDIA9 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ERP29 recombinant protein (Accession Number: NM_006817). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:110373(ERP29 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human liver slide using Catalog No:110373(ERP29 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human liver slide using Catalog No:110373(ERP29 Antibody) at dilution of 1:50
Immunofluorescent analysis of HeLa cells using Catalog No:110373(ERP29 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
IP Result of anti-ERP29 (IP:Catalog No:110373, 4ug; Detection:Catalog No:110373 1:1000) with mouse liver tissue lysate 4000ug.
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Background
Endoplasmic reticulum resident protein 29 (ERP29) is a 29-kDa endoplasmic reticulum (ER) luminal protein. Structurally ERp29 is related to protein disulfide isomerases (PDI). However, it lacks the thioredoxin motif, suggesting that this protein does not function as a disulfide isomerase (PMID: 15500441). ERP29 plays an important role in the processing of secretory proteins within the (ER), possibly by participating in the folding of proteins in the ER. The secretion of thyroglobulin (TG) was found to be strongly regulated by ERP29 (PMID: 16380091).
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