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ERK1/2 antibody

Cat.#: 110361

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Product Information

  • Product Name
    ERK1/2 antibody
  • Documents
  • Description
    ERK1/2 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A431 cells, HEK-293 cells, HepG2/A431 cells, mouse brain tissue, mouse kidney tissue, NIH/3T3 cells, PC-3 cells, rat brain tissue, rat kidney tissue. Positive IHC detected in human liver tissue, human kidney tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 38-44 kDa
  • Tested applications
    ELISA, WB, IHC, IF
  • Species reactivity
    Human,Mouse,Rat,Zebrafish; other species not tested.
  • Alternative names
    ERK antibody; ERK 2 antibody; ERK2 antibody; ERT1 antibody; MAP kinase 1 antibody; MAP kinase 2 antibody; MAP kinase isoform p42 antibody; MAPK 1 antibody; MAPK 2 antibody; MAPK1 antibody; MAPK2 antibody; p40 antibody; p41 antibody; p41mapk antibody; p42 MAPK antibody; P42MAPK antibody; PRKM1 antibody; PRKM2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of ERK1/2 recombinant protein (Accession Number: NM_002745). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:10000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110361(ERK1/2 antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110361(ERK1/2 antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human liver using Catalog No:110361(ERK1/2 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human liver using Catalog No:110361(ERK1/2 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human liver using Catalog No:110361(ERK1/2 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human liver using Catalog No:110361(ERK1/2 antibody) at dilution of 1:100 (under 40x lens)

    Immunofluorescent analysis of HeLa cells using Catalog No:110361(ERK1/2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HeLa cells using Catalog No:110361(ERK1/2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to insulin and NGF. This antibody can recognize both ERK1 and ERK2.
  • References
    • Wang CP, Li JL, Zhang LZ. Isoquercetin protects cortical neurons from oxygen-glucose deprivation-reperfusion induced injury via suppression of TLR4-NF-кB signal pathway. Neurochemistry international. 63(8):741-9. 2013.
    • Su TR, Lin JJ, Tsai CC. Inhibition of melanogenesis by gallic acid: possible involvement of the PI3K/Akt, MEK/ERK and Wnt/β-catenin signaling pathways in B16F10 cells. International journal of molecular sciences. 14(10):20443-58. 2013.
    • Zhao L, Teng B, Wen L. mTOR inhibitor AZD8055 inhibits proliferation and induces apoptosis in laryngeal carcinoma. International journal of clinical and experimental medicine. 7(2):337-47. 2014.
    • Wang CP, Zhang LZ, Li GC. Mulberroside A protects against ischemic impairment in primary culture of rat cortical neurons after oxygen-glucose deprivation followed by reperfusion. Journal of neuroscience research. 92(7):944-54. 2014.
    • Wang CP, Li GC, Shi YW. Neuroprotective effect of schizandrin A on oxygen and glucose deprivation/reperfusion-induced cell injury in primary culture of rat cortical neurons. Journal of physiology and biochemistry. 70(3):735-47. 2014.
    • Shan X, Aziz F, Tian LL, Wang XQ, Yan Q, Liu JW. Ginsenoside Rg3-induced EGFR/MAPK pathway deactivation inhibits melanoma cell proliferation by decreasing FUT4/LeY expression. International journal of oncology. 46(4):1667-76. 2015.
    • Huang P, Li C, Fu T. Flupirtine attenuates chronic restraint stress-induced cognitive deficits and hippocampal apoptosis in male mice. Behavioural brain research. 288:1-10. 2015.
    • Su C, Xia X, Shi Q. Neohesperidin Dihydrochalcone versus CCl₄-Induced Hepatic Injury through Different Mechanisms: The Implication of Free Radical Scavenging and Nrf2 Activation. Journal of agricultural and food chemistry. 63(22):5468-75. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"