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Product Name
ERCC2 antibody
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Description
ERCC2 Rabbit Polyclonal antibody. Positive IHC detected in human lymphoma tissue, human cervical cancer tissue. Positive IP detected in K-562 cells. Positive WB detected in HeLa cells, HEK-293 cells, K-562 cells. Observed molecular weight by Western-blot: 80 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
BTF2 p80 antibody; COFS2 antibody; CXPD antibody; EM9 antibody; ERCC2 antibody; TFIIH 80 kDa subunit antibody; TFIIH p80 antibody; TTD antibody; XPD antibody; XPDC antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ERCC2 recombinant protein (Accession Number: BC008346). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110408(ERCC2 antibody) at dilution of 1:500
IP Result of anti-ERCC2 (IP:Catalog No:110408, 5ug; Detection:Catalog No:110408 1:500) with K-562 cells lysate 8000ug.
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:110408(ERCC2 antibody) at dilution of 1:100 (under 10x lens)
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Background
Excision-repair complementing defective in Chinese hamster 2(ERCC2), belongs to the nucleotide excision repair pathway, is a component of the TFIIH transcription complex and required for the association of the CDK-activating kinase(CAK) subcomplex with TFIIH. Since TFIIH is involved in both DNA repair and cell cycle progression, ERCC2 is implicated to play an integral role in the nucleotide excision repair pathway. Also it involves in the regulation of vitamin-D receptor activity. As a part of the mitotic spindle-associated MMXD complex, ERCC2 has a role in chromosome segregation.
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References
- Lai JI, Tzeng CH, Chen PM. Very low prevalence of XPD K751Q polymorphism and its association with XPD expression and outcomes of FOLFOX-4 treatment in Asian patients with colorectal carcinoma. Cancer science. 100(7):1261-6. 2009.
- Zhao JY, Liu CQ, Zhao HN. Synchronous detection of miRNAs, their targets and downstream proteins in transferred FFPE sections: applications in clinical and basic research. Methods (San Diego, Calif.). 58(2):156-63. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"