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Product Name
ERC1 antibody
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Description
ERC1 Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive WB detected in HeLa cells, COLO 320 cells, human testis tissue, Jurkat cells. Observed molecular weight by Western-blot: 124-135 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
Cast2 antibody; ELKS antibody; ERC 1 antibody; ERC1 antibody; KIAA1081 antibody; Rab6 interacting protein 2 antibody; RAB6IP2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ERC1 recombinant protein (Accession Number: XM_011520941). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110405(ERC1 antibody) at dilution of 1:1000
Immunofluorescent analysis of MCF-7 cells, using ERC1 antibody Catalog No:110405 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
ERC is an acronym based on previous separate namings of members of this protein family, ELKS, Rab6IP2 and CAST (PMID: 12391317). They are known to bind RIMs, the active zone proteins that regulate neurotransmitter release. ERC1 (ELKS) is an essential regulatory subunit of the IKK complex and likely functions by recruiting IκBα to the complex (PMID: 15218148). Multiple isoforms of ERC1 mRNA are generated by alternative splicing (PMID: 12203787). There are two known splice variants of ERC1 protein that differ at their C termini, ERC1a and ERC1b. ERC1a is ubiquitously expressed, whereas ERC1b is brain-specific (PMID: 12391317; 12923177).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"