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Product Name
ERAB antibody
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Description
ERAB Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A2780 cells, human brain tissue, human liver tissue, mouse liver tissue, Transfected cells, Transfected HEK-293 cells. Positive IP detected in HeLa cells. Positive FC detected in MCF-7 cells. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 27 kDa
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Tested applications
ELISA, WB, IHC, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
17 beta HSD 10 antibody; 17b HSD10 antibody; ABAD antibody; CAMR antibody; DUPXp11.22 antibody; ERAB antibody; HADH2 antibody; HCD2 antibody; HSD17B10 antibody; MHBD antibody; MRPP2 antibody; MRX17 antibody; MRX31 antibody; MRXS10 antibody; SCHAD antibody; SDR5C1 antibody; Type II HADH antibody; XH98G2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ERAB recombinant protein (Accession Number: NM_001037811). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110398(HSD17B10 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:110398(HSD17B10 antibody) at dilution of 1:100 (under 10x lens)
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:110398(HSD17B10 Antibody) at dilution of 1:1000
IP Result of anti-HSD17B10 (IP:Catalog No:110398, 4ug; Detection:Catalog No:110398 1:500) with HeLa cells lysate 1200ug.
1X10^6 MCF-7 cells were stained with 0.2ug HSD17B10 antibody (Catalog No:110398, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
HSD17B10 (3-hydroxyacyl-CoA dehydrogenase type-2) is a multifunctional mitochondrial enzyme that acts on a wide spectrum of substrates, including neuroactive steroids, alcohols, leucine, and fatty acids, with a preference for short-chain methyl-branched acyl-CoAs(PMID:15860413).It has 2 isoforms produced by alternative splicing.Defects in HSD17B10 are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD deficiency) and mental retardation syndromic X-linked type 10 (MRXS10).
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