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Product Name
EPS8 antibody
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Description
EPS8 Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, A375 cells, HeLa cells, human heart tissue. Positive IP detected in HeLa cells. Positive IHC detected in human breast cancer tissue. Positive IF detected in A375 cells. Observed molecular weight by Western-blot: 97 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human; other species not tested.
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Alternative names
EPS8 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EPS8 recombinant protein (Accession Number: XM_024448882). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110394(EPS8 antibody) at dilution of 1:500
IP Result of anti-EPS8 (IP:Catalog No:110394, 3ug; Detection:Catalog No:110394 1:500) with HeLa cells lysate 3800ug.
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:110394(EPS8 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of A375 cells using Catalog No:110394(EPS8 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Epidermal growth factor receptor Pathway Substrate 8 (EPS8) is a crucial regulator of the actin cytoskeleton dynamics accompanying cell motility and invasion. This protein contains one PH domain and one SH3 domain leading to its binding activity with multiple cellular targets. EPS8 can function as a unique actin capping protein specifically required for dendritic cell migration and plays roles in the regulation of axonal filopodia in neuronal development and synapse formation. The EPS8 gene may contribute to the development of a subset of colorectal cancers and could have applications in diagnosis and treatment.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"