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Product Name
EPLIN antibody
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Description
EPLIN Mouse Monoclonal antibody. Positive WB detected in Colo320 cells, HEK-293 cells, HeLa cells, human brain tissue. Positive IHC detected in human heart tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 90kD,110kD
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
EPLIN antibody; LIM domain and actin binding 1 antibody; LIMA1 antibody; SREBP3 antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of EPLIN recombinant protein (Accession Number: BC001247). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:107191(LIMA1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human heart using Catalog No:107191(LIMA1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:107191(LIMA1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using LIMA1 antibody Catalog No: at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
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Background
LIMA1 represents LIM domain and actin binding 1
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"