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Product Name
EMG1 antibody
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Description
EMG1 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, HEK-293 cells, PC-3 cells. Observed molecular weight by Western-blot: 27 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C2F antibody; EMG1 antibody; FLJ60792 antibody; Grcc2f antibody; NEP1 antibody; Nucleolar protein EMG1 homolog antibody; Protein C2f antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EMG1 recombinant protein (Accession Number: NM_006331). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110238(EMG1 antibody) at dilution of 1:400
IP Result of anti-EMG1 (IP:Catalog No:110238, 4ug; Detection:Catalog No:110238 1:400) with HeLa cells lysate 1080ug.
Immunofluorescent analysis of HeLa cells using Catalog No:110238(EMG1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
EMG1, also named as NEP1, is a highly conserved protein initially identified as “Essential for Mitotic Growth” in yeast. EMG1 is required for maturation of the 18S rRNA and biogenesis of the 40S ribosomal subunit. EMG1 is the methyltransferase in the biosynthesis of m1acp3-Psi in eukaryotic 18S rRNAs. It has an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity. A mutation in human EMG1 gene causes Bowen-Conradi syndrome (BCS), an autosomal recessive disorder characterized by severely impaired prenatal and postnatal growth, profound psychomotor retardation, and death in early childhood.
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References
- Armistead J, Patel N, Wu X. Growth arrest in the ribosomopathy, Bowen-Conradi syndrome, is due to dramatically reduced cell proliferation and a defect in mitotic progression. Biochimica et biophysica acta. 1852(5):1029-37. 2015.
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