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Product Name
ELL antibody
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Description
ELL Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, HL-60 cells, mouse testis tissue. Positive IHC detected in human ovary tumor. Observed molecular weight by Western-blot: 68-80kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
human, mouse; other species not tested.
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Alternative names
ELL antibody; C19orf17 antibody; DKFZp434I1916 antibody; MEN antibody; ELL1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ELL recombinant protein (Accession Number: NM_006532). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110280(ELL antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:110280(ELL antibody) at dilution of 1:50 (under 10x lens)
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Background
ELL is an elongation factor that inrecase the catalytic rate of RNA polymerase II transcription via suppressing transient pausing by the polymerase at multiple sites along the DNA. Also it's the second elongation factor to be involved in oncogenesis which is a transcription factor regulated by the product of the von Hippel-Lindau tumor suppressor. There are two transcirpts variants in mRNA level, one is 4.4kb transcript and the other is 2.8kb transcript.
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References
- Liu L, Ai J, Xiao W. ELL is an HIF-1alpha partner that regulates and responds to hypoxia response in PC3 cells. The Prostate. 70(7):797-805. 2010.
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