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Product Name
EIF5B antibody
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Description
EIF5B Rabbit Polyclonal antibody. Positive IHC detected in human gliomas. Positive IF detected in MCF-7. Positive WB detected in mouse brain tissue, A549 cells. Observed molecular weight by Western-blot: 175kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
EIF5B antibody; eIF 5B antibody; IF2 antibody; DKFZp434I036 antibody; FLJ10524 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EIF5B recombinant protein (Accession Number: NM_015904). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110269(EIF5B antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:110269(EIF5B antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:110269(EIF5B antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using EIF5B antibody Catalog No:110269 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
Translation initiation requires the delivery of the initiator methionine tRNA to the 40S ribosomal subunit. The initiator methionine tRNA is delivered by the heterotrimeric complex EIF2 in a ternary complex with GTP that interacts with the 40S subunit. The resulting complex then binds to an mRNA and scans for the AUG start codon. Eukaryotic translation initiation factor 5B (EIF5B) plays a role in recognition of the AUG codon in conjunction with translation factor eIF2, which functions to general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes, and promotes joining of the 60S ribosomal subunit.
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