-
Product Name
EIF4A3 antibody
- Documents
-
Description
EIF4A3 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, human lung tissue, mouse liver tissue, Raji cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 47kd
-
Tested applications
ELISA, WB, IP
-
Species reactivity
Human, Mouse; other species not tested.
-
Alternative names
DDX48 antibody; DEAD box protein 48 antibody; eIF 4A III antibody; eIF4A III antibody; EIF4A3 antibody; eIF4AIII antibody; hNMP 265 antibody; hNMP265 antibody; KIAA0111 antibody; Nuclear matrix protein 265 antibody; NUK 34 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of EIF4A3 recombinant protein (Accession Number: NM_014740). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110258(EIF4A3 antibody) at dilution of 1:1000
IP Result of anti-EIF4A3 (IP:Catalog No:110258, 3ug; Detection:Catalog No:110258 1:300) with HEK-293 cells lysate 2800ug.
-
Background
EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions
-
References
- Yamashita A, Izumi N, Kashima I. SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay. Genes & development. 23(9):1091-105. 2009.
- Izumi N, Yamashita A, Iwamatsu A. AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK activity and function in nonsense-mediated mRNA decay. Science signaling. 3(116):ra27. 2010.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"