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Product Name
EIF4A2 antibody
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Description
EIF4A2 Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells, HepG2 cells. Positive IHC detected in human ovary tumor tissue, human breast cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 46kd
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BM 010 antibody; DDX2B antibody; eIF 4A II antibody; EIF4A antibody; eIF4A II antibody; EIF4A2 antibody; EIF4F antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EIF4A2 recombinant protein (Accession Number: NM_001967). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:110201(EIF4A2 antibody) at dilution of 1:500
Immunofluorescent analysis of HepG2 cells, using EIF4A2 antibody Catalog No:110201 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:110201(EIF4A2 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:110201(EIF4A2 Antibody) at dilution of 1:50 (under 40x lens)
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Background
Eukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.
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