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Product Name
EIF3J antibody
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Description
EIF3J Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A549 cells, HEK-293 cells, Jurkat cells, K-562 cells, MCF7 cells, SKOV-3 cells. Positive IP detected in HeLa cells. Positive IF detected in Hela cells. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 35 kDa, 60-66 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
eIF 3 alpha antibody; eIF3 alpha antibody; eIF3 p35 antibody; EIF3J antibody; EIF3S1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EIF3J recombinant protein (Accession Number: NM_003758). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110198(EIF3J antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:110198(EIF3J antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of Hela cells, using EIF3J antibody Catalog No:110198 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-EIF3J (IP:Catalog No:110198, 4ug; Detection:Catalog No:110198 1:500) with HeLa cells lysate 880ug.
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Background
EIF3 has a key role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex(PMID:17588516). The eIF3 complex stimulates several steps in the translation initiation pathway, including dissociation of 80S ribosomes into 40S and 60S subunits, binding of a ternary complex (TC) consisting of Met-tRNA , eIF2, and GTP to the small subunit (forming the 43S preinitiation complex) and recruitment of mRNA to the 43 S complex to produce the 48S complex (PMID:11560931) EIF3J was identified as a high copy suppressor of the temperature-sensitive (Ts-) phenotype of the rpg1-1 allele of TIF32/RPG1, encoding the largest subunit of yeast eIF3 (eIF3a) (PMID:10488093).
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References
- Ramírez-Valle F, Braunstein S, Zavadil J, Formenti SC, Schneider RJ. eIF4GI links nutrient sensing by mTOR to cell proliferation and inhibition of autophagy. The Journal of cell biology. 181(2):293-307. 2008.
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