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Product Name
EIF3E antibody
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Description
EIF3E Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells. Positive IF detected in HepG2 cells, Ethacrynic acid treated HepG2 cells. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 48 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
eIF 3 p48 antibody; eIF3 p46 antibody; EIF3 P48 antibody; EIF3E antibody; EIF3S6 antibody; INT6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EIF3E recombinant protein (Accession Number: NM_001568). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110194(EIF3E antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:110194(EIF3E antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:110194(EIF3E Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
EIF3E represents eukaryotic translation initiation factor 3, subunit E
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"