Cart 2

EIF2S3 antibody

Cat.#: 110189

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    EIF2S3 antibody
  • Documents
  • Description
    EIF2S3 Rabbit Polyclonal antibody. Positive IHC detected in human lung cancer tissue, human tonsillitis tissue. Positive IF detected in HeLa cells. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, COLO 320 cells, mouse thymus tissue. Observed molecular weight by Western-blot: 52 kDa
  • Tested applications
    ELISA, WB, IHC, IP, IF
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    eIF 2 gamma X antibody; eIF 2gA antibody; eIF 2gX antibody; EIF2 antibody; EIF2G antibody; EIF2gamma antibody; EIF2S3 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of EIF2S3 recombinant protein (Accession Number: NM_001415). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:50-1:500

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110189(EIF2S3 antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110189(EIF2S3 antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:110189(EIF2S3 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:110189(EIF2S3 antibody) at dilution of 1:50 (under 10x lens)

    IP Result of anti-EIF2S3 (IP:Catalog No:110189, 4ug; Detection:Catalog No:110189 1:500) with HeLa cells lysate 2800ug.

    IP Result of anti-EIF2S3 (IP:Catalog No:110189, 4ug; Detection:Catalog No:110189 1:500) with HeLa cells lysate 2800ug.

    Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:110189(EIF2S3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:110189(EIF2S3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Interacts with GTP and initiator methionyl-tRNA, translation initiation factor eIF2 forms a ternary complex that binds the 40S ribosome and then scans an mRNA to select the AUG start codon for protein synthesis[PMID: 9736774]. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2S3 is the core subunit of the heterotrimeric eIF2 complex.[PMID:23063529]
  • References
    • Stoll G, Pietiläinen OP, Linder B. Deletion of TOP3β, a component of FMRP-containing mRNPs, contributes to neurodevelopmental disorders. Nature neuroscience. 16(9):1228-37. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"