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Product Name
EIF2C1-Specific antibody
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Description
EIF2C1-Specific Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human cervical cancer tissue. Observed molecular weight by Western-blot: 97 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AGO1 antibody; Argonaute1 antibody; DKFZp686M13167 antibody; eIF 2C 1 antibody; EIF2C antibody; eIF2C 1 antibody; EIF2C1 antibody; GERP95 antibody; hAgo1 antibody; Protein argonaute 1 antibody; Q99 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_012199). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110185(EIF2C1-Specific antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:110185(EIF2C1-Specific antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:110185(EIF2C1-Specific Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
EIF2C1, also named as AGO1, belongs to the argonaute family and Ago subfamily. EIF2C1 is required for RNA-mediated gene silencing (RNAi). It binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. EIF2C1 may also be required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). The antibody is specific to EIF2C1.
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