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EIF2B1 antibody

Cat.#: 110181

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Special Price 239.0 USD

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Product Information

  • Product Name
    EIF2B1 antibody
  • Documents
  • Description
    EIF2B1 Rabbit Polyclonal antibody. Positive IP detected in K-562 cells. Positive WB detected in MCF7 cells, HeLa cells, K-562 cells. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 34 kDa
  • Tested applications
    ELISA, WB, IF, IP
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    EIF 2B antibody; EIF 2Balpha antibody; EIF2B antibody; EIF2B1 antibody; EIF2BA antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of EIF2B1 recombinant protein (Accession Number: NM_001414). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IF: 1:10-1:100

  • Validations

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:110181(EIF2B1 antibody) at dilution of 1:500

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:110181(EIF2B1 antibody) at dilution of 1:500

    Immunofluorescent analysis of Hela cells, using EIF2B1 antibody Catalog No:110181 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of Hela cells, using EIF2B1 antibody Catalog No:110181 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    IP Result of anti-EIF2B1 (IP:Catalog No:110181, 4ug; Detection:Catalog No:110181 1:700) with K-562 cells lysate 3200ug.

    IP Result of anti-EIF2B1 (IP:Catalog No:110181, 4ug; Detection:Catalog No:110181 1:700) with K-562 cells lysate 3200ug.

  • Background
    EIF2B1 represents eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa
  • References
    • Wortham NC, Martinez M, Gordiyenko Y, Robinson CV, Proud CG. Analysis of the subunit organization of the eIF2B complex reveals new insights into its structure and regulation. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 28(5):2225-37. 2014.
    • Sidrauski C, Tsai JC, Kampmann M. Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response. eLife. 4:e07314. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"