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Product Name
EIF2AK2, PKR antibody
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Description
EIF2AK2, PKR Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive IHC detected in human testis tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue. Positive WB detected in MCF7 cells, HeLa cells, Jurkat cells, rat heart tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 65 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
eIF 2A protein kinase 2 antibody; EIF2AK1 antibody; EIF2AK2 antibody; P1/eIF 2A protein kinase antibody; p68 kinase antibody; PKR antibody; PRKR antibody; Protein kinase RNA activated antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EIF2AK2, PKR recombinant protein (Accession Number: NM_002759). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:110180(EIF2AK2 antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human testis using Catalog No:110180(EIF2AK2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:110180(EIF2AK2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using EIF2AK2 antibody Catalog No:110180 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-EIF2AK2 (IP:Catalog No:110180, 4ug; Detection:Catalog No:110180 1:500) with HeLa cells lysate 1200ug.
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Background
The eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2, also known as PKR or PRKR) is an important component of the host innate immuneantiviral response.The product of the HSAEIF2AK2 gene is a 68 kDa protein that is ubiquitously expressed at low levels. The N-terminal part of this protein contains two dsRNA binding motifs (dsRBM) .The C-terminal EIF2S1 kinase domain includes 12 sub-domains that are conserved in several Species(PMID:16954046 ).This antibody is specific to EIF2AK2.
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References
- Wu H, Wei L, Fan F. Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis. Nature communications. 6:6239. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"