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Product Name
EIF2AK1 antibody
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Description
EIF2AK1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in HeLa cells, HepG2 cells. Observed molecular weight by Western-blot: 71kd
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
EIF2AK1 antibody; HCR antibody; Heme controlled repressor antibody; Heme regulated inhibitor antibody; HRI antibody; KIAA1369 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EIF2AK1 recombinant protein (Accession Number: NM_014413). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110179(EIF2AK1 antibody) at dilution of 1:500
Immunofluorescent analysis of HepG2 cells, using EIF2AK1 antibody Catalog No:110179 at 1:25 dilution and FITC- labeled goat anti-Rabbit IgG (green).
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Background
Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions.
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References
- Wu H, Wei L, Fan F. Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis. Nature communications. 6:6239. 2015.
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