-
Product Name
EIF2A antibody
- Documents
-
Description
EIF2A Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, HeLa cells, MCF7 cells, MDA-MB-453s cells, mouse pancreas tissue, PC-3 cells, Raji cells. Positive IP detected in MCF-7 cells. Positive IF detected in HepG2 cells, Ethacrynic acid treated HepG2 cells. Positive IHC detected in human prostate cancer tissue, human pancreas tissue. Positive FC detected in HepG2 cells. Observed molecular weight by Western-blot: 65 kDa
-
Tested applications
ELISA, WB, IHC, IP, IF, FC
-
Species reactivity
Human, Mouse, Rat; other species not tested.
-
Alternative names
CDA02 antibody; EIF 2A antibody; EIF2A antibody; MST089 antibody; MSTP004 antibody; MSTP089 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of EIF2A recombinant protein (Accession Number: NM_032025). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:110178(EIF2A antibody) at dilution of 1:300
IP Result of anti-EIF2A (IP:Catalog No:110178, 3ug; Detection:Catalog No:110178 1:1000) with MCF-7 cells lysate 3250ug.
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:110178(EIF2A antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using EIF2A antibody Catalog No:110178 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
1X10^6 HepG2 cells were stained with .2ug EIF2A antibody (Catalog No:110178, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
-
Background
EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human EIF2A, recognizes specifically the 65kd EIF2A protein.
-
References
- Kim JH, Park SM, Park JH, Keum SJ, Jang SK. eIF2A mediates translation of hepatitis C viral mRNA under stress conditions. The EMBO journal. 30(12):2454-64. 2011.
- Starck SR, Jiang V, Pavon-Eternod M. Leucine-tRNA initiates at CUG start codons for protein synthesis and presentation by MHC class I. Science (New York, N.Y.). 336(6089):1719-23. 2012.
- Dong D, Zhang S, Yin L. Protective effects of the total saponins from Rosa laevigata Michx fruit against carbon tetrachloride-induced acute liver injury in mice. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 62:120-30. 2013.
- Xu T, Zheng L, Xu L. Protective effects of dioscin against alcohol-induced liver injury. Archives of toxicology. 88(3):739-53. 2014.
- Lin CJ, Robert F, Sukarieh R, Michnick S, Pelletier J. The antidepressant sertraline inhibits translation initiation by curtailing mammalian target of rapamycin signaling. Cancer research. 70(8):3199-208. 2010.
- Xu D, Su C, Song X. Polychlorinated biphenyl quinone induces endoplasmic reticulum stress, unfolded protein response, and calcium release. Chemical research in toxicology. 28(6):1326-37. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"