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Product Name
EGFR (C-terminal) antibody
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Description
EGFR (C-terminal) Rabbit Polyclonal antibody. Positive IP detected in A431 cells. Positive WB detected in A431 cells, HeLa cells. Positive IF detected in HeLa cells. Positive IHC detected in human skin cancer tissue. Observed molecular weight by Western-blot: 170 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human; other species not tested.
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Alternative names
EGFR antibody; ERBB antibody; ERBB1 antibody; HER1 antibody; mENA antibody; PIG61 antibody; Proto oncogene c ErbB 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001346897). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:110216(EGFR antibody) at dilution of 1:800
IP Result of anti-EGFR (IP:Catalog No:110216, 5ug; Detection:Catalog No:110216 1:500) with A431 cells lysate 3000ug.
Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:110216(EGFR antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:110216(EGFR antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:110216(EGFR Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer. The antibody is specific to isoform1.
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