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Product Name
EFTUD2 antibody
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Description
EFTUD2 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in HeLa cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 116 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp686E24196 antibody; EFTUD2 antibody; FLJ44695 antibody; hSNU114 antibody; KIAA0031 antibody; Snrp116 antibody; Snu114 antibody; SNU114 homolog antibody; U5 116 kDa antibody; U5 116KD antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EFTUD2 recombinant protein (Accession Number: NM_001258353). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110213(EFTUD2 antibody) at dilution of 1:1000
IP Result of anti-EFTUD2 (IP:Catalog No:110213, 3ug; Detection:Catalog No:110213 1:1000) with HeLa cells lysate 4650ug.
Immunofluorescent analysis of HepG2 cells, using EFTUD2 antibody Catalog No:110213 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
EFTUD2 encodes the 116 kDa U5 small nuclear ribonecleoprotein (snRNP) component, also named SNU114, it has GTP binding activity and is required for pre-mRNA splicing. Evolutionarily conserved human snRNP protein (U5-116kDa) is homologous to the ribosomal elongation factor EF-2 (ribosomal translocase). Defects in EFTUD2 are the cause of mandibulofacial dysostosis with microcephaly (MFDM). So far, acetylation and phosphorylation sites of EFTUD2 have been identified. Catolog#10208-1-AP is a rabbit polyclonal antibody raised against the N-terminal of human EFTUD2.
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References
- Murray J, Huss JM. Estrogen-related receptor α regulates skeletal myocyte differentiation via modulation of the ERK MAP kinase pathway. American journal of physiology. Cell physiology. 301(3):C630-45. 2011.
- Berg MG, Wan L, Younis I. A quantitative high-throughput in vitro splicing assay identifies inhibitors of spliceosome catalysis. Molecular and cellular biology. 32(7):1271-83. 2012.
- Huang R, Yu M, Li CY. New insights into the functions and localization of nuclear CCT protein complex in K562 leukemia cells. Proteomics. Clinical applications. 6(9-10):467-75. 2012.
- Szerlong HJ, Herman JA, Krause CM. Proteomic characterization of the nucleolar linker histone H1 interaction network. Journal of molecular biology. 427(11):2056-71. 2015.
- Jang MK, Anderson DE, van Doorslaer K, McBride AA. A proteomic approach to discover and compare interacting partners of papillomavirus E2 proteins from diverse phylogenetic groups. Proteomics. 15(12):2038-50. 2015.
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