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Product Name
ECE2 antibody
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Description
ECE2 Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in mouse liver tissue, mouse lung tissue, mouse ovary tissue. Observed molecular weight by Western-blot: 100 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ECE2 antibody; endothelin converting enzyme 2 antibody; KIAA0604 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ECE2 recombinant protein (Accession Number: BC005835). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:110291(ECE2 antibody) at dilution of 1:500
IP Result of anti-ECE2 (IP:Catalog No:110291, 4ug; Detection:Catalog No:110291 1:500) with mouse liver tissue lysate 4000ug.
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Background
Endothelin-converting enzymes (ECEs) are the key enzymes in the endothelin (ET) biosynthesis that catalyze the conversion of big ET, the biologically inactive precursor of mature ET. Two enzymes, termed ECE-1 and ECE-2, have been molecularly identified. ECE2(Endothelin-converting enzyme 2) is also named as KIAA0604 and belongs to the methyltransferase superfamily in the N-terminal section, belongs to the peptidase M13 family in the C-terminal section. It is able to process endoproteolytically peptides intermediates to generate biologically active peptides.
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References
- Mitchell AJ, Lone AM, Tinoco AD, Saghatelian A. Proteolysis controls endogenous substance P levels. PloS one. 8(7):e68638. 2013.
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