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Product Name
ECD antibody
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Description
ECD Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 73 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
ECD antibody; GCR2 antibody; HSGT1 antibody; Protein ecdysoneless homolog antibody; Protein SGT1 antibody; SGT1 antibody; Suppressor of GCR2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ECD recombinant protein (Accession Number: NM_007265). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110290(ECD antibody) at dilution of 1:300
IP Result of anti-ECD (IP:Catalog No:110290, 4ug; Detection:Catalog No:110290 1:300) with HeLa cells lysate 2480ug.
Immunofluorescent analysis of HeLa cells using Catalog No:110290(ECD Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The human suppressor of GCR two (hSGT1) was isolated by its ability to complement the growth defect of the gcr2 mutant of Saccharomyces cerevisiae. Further work confirmed that the rescue of growth was associated with recovery of glycolytic enzyme activities of gcr2, and that hSGT1 did not complement the growth defect of a gcr1 mutant. A hybrid protein comprising hSgt1p and the DNA-binding domain of Gal4p (GBD) activated a GAL1-lacZ reporter gene fusion, suggesting that the cloned gene may be a transcriptional activator. Two-hybrid experiments in yeast also indicate that hSgt1p interacts with Gcr1p. Northern analysis showed that hSGT1 is highly expressed in muscle and heart.
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References
- Xu SH, Huang JZ, Chen M. Amplification of ACK1 promotes gastric tumorigenesis via ECDdependent p53 ubiquitination degradation. Oncotarget. 2015.
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