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Product Name
EB1 antibody
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Description
EB1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A431 cells, Jurkat cells. Positive IP detected in HeLa cells, mouse thymus tissue. Positive IHC detected in human kidney tissue, human colon cancer tissue, human lung tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue. Observed molecular weight by Western-blot: 35 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
APC binding protein EB1 antibody; EB1 antibody; End binding protein 1 antibody; MAPRE1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EB1 recombinant protein (Accession Number: NM_012325). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110165(MAPRE1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:110165(MAPRE1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:110165(MAPRE1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-EB1 (IP:Catalog No:110165, 4ug; Detection:Catalog No:110165 1:1000) with HeLa cells lysate 1200ug.
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Background
MAPRE1 represents microtubule-associated protein, RP/EB family, member 1
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