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Product Name
E2F3 antibody
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Description
E2F3 Rabbit Polyclonal antibody. Positive WB detected in Y79 cells, HEK-293 cells, HepG2 cells, mouse liver tissue. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 52 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp686C18211 antibody; E2F 3 antibody; E2F transcription factor 3 antibody; E2F3 antibody; KIAA0075 antibody; Transcription factor E2F3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of E2F3 recombinant protein (Accession Number: BC016847). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:110154(E2F3 Antibody) at dilution of 1:600
IP Result of anti-E2F3 (IP:Catalog No:110154, 4ug; Detection:Catalog No:110154 1:500) with mouse liver tissue lysate 4000ug.
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Background
E2F transcription factors regulate gene expression in concert with the retinoblastoma tumor suppressor family. These transcriptional complexes are master regulators of cell cycle progression, control the expression of genes involved in DNA repair, G2/M checkpoint and differentiation [PMID:22580460]. E2f3 is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner [PMID:22960857].
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References
- Xu D, Wang J, Zhou Z, He Z, Zhao Q. Cannabinoid WIN55, 212-2 induces cell cycle arrest and inhibits the proliferation and migration of human BEL7402 hepatocellular carcinoma cells. Molecular medicine reports. 12(6):7963-70. 2015.
- Wang B, Ma A, Zhang L. POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation. Nature communications. 6:8704. 2015.
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