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Product Name
DYNC1H1 antibody
- Documents
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Description
DYNC1H1 Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive IHC detected in human breast cancer tissue, human testis tissue. Positive WB detected in HeLa cells, human brain tissue, mouse brain tissue. Observed molecular weight by Western-blot: 532kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Rat, Zebrafish, Mouse; other species not tested.
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Alternative names
DHC1 antibody; DHC1a antibody; DKFZp686P2245 antibody; DNCH1 antibody; Dnchc1 antibody; DNCL antibody; DNECL antibody; DYHC antibody; DYNC1H1 antibody; Dynein heavy chain antibody; cytosolic antibody; HL 3 antibody; KIAA0325 antibody; p22 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DYNC1H1 recombinant protein (Accession Number: BC021297). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110134(KIAA0325 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:110134(KIAA0325 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells, using DYNC1H1 antibody Catalog No:110134 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
Dyneins are a group of microtubule-activated ATPases that serve to convert chemical energy into mechanical energy. It can be divided into 2 large subgroups, namely, the axonemal and cytoplasmic dyneins. The conventional cytoplasmic dynein are comprised of 2 heavy chain polypeptides and a number of intermediate and light chains. DYNC1H1 is a cytoplasmic dynein and belongs to the dynein heavy chain family. It acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. DYNC1H1 has been implicated in the degeneration of dopaminergic neuron axons and motor neurons in PD patients..
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References
- Renard HF, Simunovic M, Lemière J. Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis. Nature. 517(7535):493-6. 2015.
- Insinna C, Baye LM, Amsterdam A, Besharse JC, Link BA. Analysis of a zebrafish dync1h1 mutant reveals multiple functions for cytoplasmic dynein 1 during retinal photoreceptor development. Neural development. 5:12. 2010.
- Zhao X, Xiao WZ, Pu XP, Zhong LJ. Proteome analysis of the sera from Chinese Parkinson's disease patients. Neuroscience letters. 479(2):175-9. 2010.
- Mu R, Wang YB, Wu M. Depletion of pre-mRNA splicing factor Cdc5L inhibits mitotic progression and triggers mitotic catastrophe. Cell death & disease. 5:e1151. 2014.
- Schroeder CM, Ostrem JM, Hertz NT, Vale RD. A Ras-like domain in the light intermediate chain bridges the dynein motor to a cargo-binding region. eLife. 3:e03351. 2014.
- Gholkar AA, Senese S, Lo YC. Tctex1d2 associates with short-rib polydactyly syndrome proteins and is required for ciliogenesis. Cell cycle (Georgetown, Tex.). 14(7):1116-25. 2015.
- Inaba H, Goto H, Kasahara K. Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway. The Journal of cell biology. 212(4):409-23. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"