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Product Name
DVL2 antibody
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Description
DVL2 Rabbit Polyclonal antibody. Positive WB detected in NIH/3T3 cells, HepG2 cells, MCF-7 cells, MDA-MB-453s cells, mouse thymus tissue. Positive IP detected in MCF-7 cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 90 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Dishevelled 2 antibody; DSH homolog 2 antibody; DVL2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DVL2 recombinant protein (Accession Number: NM_004422). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:20-1:200
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Validations
WB result of DVL2 antibody (Catalog No:110125, 1:1000) with siDVL2 transfected cells.
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:110125(DVL2 Antibody) at dilution of 1:1000
IP Result of anti-DVL2 (IP:Catalog No:110125, 4ug; Detection:Catalog No:110125 1:1000) with MCF-7 cells lysate 3200ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:110125(DVL2 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG
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Background
DVL2 is member of the dishevelled (dsh) protein family. Dvl2 participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. It promotes internalization and degradation of frizzled proteins upon Wnt signaling. It plays a role both in canonical and non-canonical Wnt signaling. It also Plays a role in the signal transduction pathways mediated by multiple Wnt genes. This antibody is a specific one for siRNA detection.
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References
- Gentzel M, Schille C, Rauschenberger V, Schambony A. Distinct functionality of dishevelled isoforms on Ca2+/calmodulin-dependent protein kinase 2 (CamKII) in Xenopus gastrulation. Molecular biology of the cell. 26(5):966-77. 2015.
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