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Product Name
DTYMK antibody
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Description
DTYMK Rabbit Polyclonal antibody. Positive FC detected in MCF-7 cells. Positive IHC detected in human colon cancer tissue. Positive IF detected in MCF-7 cells, HepG2 cells. Positive WB detected in HeLa cells, HepG2 cells, human brain tissue, human heart tissue, mouse brain tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 24kd
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Tested applications
ELISA, WB, IHC, IF, FC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CDC8 antibody; dTMP kinase antibody; DTYMK antibody; FLJ44192 antibody; Thymidylate kinase antibody; TMPK antibody; TYMK antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DTYMK recombinant protein (Accession Number: NM_012145). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110041(DTYMK antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:110041(DTYMK antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:110041(DTYMK antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using DTYMK antibody Catalog No:110041 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-DTYMK (IP:Catalog No:110041, 3ug; Detection:Catalog No:110041 1:1000) with HeLa cells lysate 2800ug.
1X10^6 MCF-7 cells were stained with 0.2ug DTYMK antibody (Catalog No:110041, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
DTYMK(Thymidylate kinase) is also named as CDC8,TMPK and TYMK.It catalyzes the phosphorylation of dTMP to form dTDP in the dTTP synthesis pathway for DNA synthesis.This enzyme is important in cellular DNA synthesis because the synthesis of dTTP either via the de novo pathway or through the exogenous supply of thymidine requires the activity of this enzyme(PMID:18469).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"