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Product Name
DSG2 antibody
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Description
DSG2 Rabbit Polyclonal antibody. Positive IF detected in A431 cells. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells, A431 cells, A549 cells, HEK-293 cells, HeLa cells. Observed molecular weight by Western-blot: 145-150 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ARVC10 antibody; ARVD10 antibody; Cadherin family member 5 antibody; CDHF5 antibody; desmoglein 2 antibody; DSG2 antibody; HDGC antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DSG2 recombinant protein (Accession Number: NM_001943). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:110027(DSG2 antibody) at dilution of 1:1000
IP Result of anti-DSG2 (IP:Catalog No:110027, 4ug; Detection:Catalog No:110027 1:1000) with HepG2 cells lysate 3600ug.
Immunofluorescent analysis of A431 cells using Catalog No:110027(DSG2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG2 is a single-pass transmembrane glycoprotein that is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis, and heart (PMID:21715983). Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10), and genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB).
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References
- Zhang C, Zhang H, Yu L, Cao Y. Helicobacter pylori dwelling on the apical surface of gastrointestinal epithelium damages the mucosal barrier through direct contact. Helicobacter. 19(5):330-42. 2014.
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