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Product Name
DSE antibody
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Description
DSE Mouse Monoclonal antibody. Positive IHC detected in human liver cancer tissue. Positive WB detected in COLO 320 cells. Observed molecular weight by Western-blot: 100 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
dermatan sulfate epimerase antibody; DS epimerase antibody; DSE antibody; DSEPI antibody; SART 2 antibody; SART2 antibody
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Isotype
Mouse IgM
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Preparation
This antibody was obtained by immunization of DSE recombinant protein (Accession Number: NM_013352). Purification method: Caprylic acid/ammonium sulfate precipitation.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:107242(DSE antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:107242(DSE Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:107242(DSE Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Background
DSE, also named as SART2 and DSEPI, is an enzyme that converts D-glucuronic acid to L-iduronic acid residues in dermatan sulphate biosynthesis. It is also identified to be a tumour-associated antigen. DSE is recognized by cytotoxic T cells (CTLs) and its enhanced expression in many cancers has been reported. DSE is a potential candidate for a tumour antigen with immunogenicity.
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