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Product Name
DOCK7 antibody
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Description
DOCK7 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in human brain tissue, mouse brain tissue, mouse ovary tissue. Observed molecular weight by Western-blot: 238-243 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
dedicator of cytokinesis 7 antibody; DOCK7 antibody; KIAA1771 antibody; ZIR2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of D℃K7 recombinant protein (Accession Number: BC016392). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:2000
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110046(DocK7 antibody) at dilution of 1:300
IP Result of anti-DocK7 (IP:Catalog No:110046, 3ug; Detection:Catalog No:110046 1:500) with mouse brain tissue lysate 8000ug.
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Background
DocK 7 (dedicator of cytokinesis 7), also known as ZIR2, is a member of the DocK180-related protein superfamily. Expressed mainly in neuronal cells, DocK 7 is a guanine nucleotide exchange factor (GEF) for small GTPases, Rac1 and Cdc42, which are the major regulators of actin cytoskeleton. Multiple isoforms of DocK 7 exist due to alternative splicing events. The DocK 7 antibody (13000-1-AP) can recognize all the isoforms.
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References
- Ryder PV, Vistein R, Gokhale A, Seaman MN, Puthenveedu MA, Faundez V. The WASH complex, an endosomal Arp2/3 activator, interacts with the Hermansky-Pudlak syndrome complex BLOC-1 and its cargo phosphatidylinositol-4-kinase type IIα. Molecular biology of the cell. 24(14):2269-84. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"