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Product Name
DNTTIP1 antibody
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Description
DNTTIP1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 37 kDa
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Tested applications
ELISA, IP, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C20orf167 antibody; dJ447F3.4 antibody; DNTTIP1 antibody; Tdif1 antibody; TdT interacting factor 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DNTTIP1 recombinant protein (Accession Number: NM_052951). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IF: 1:50-1:500
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Validations
HeLa cell were subjected to SDS PAGE followed by western blot with Catalog No:109973 (DNTTIP1 Antibody) at dilution of 1:500
IP Result of anti-DNTTIP1 (IP:Catalog No:109973, 4ug; Detection:Catalog No:109973 1:300) with HeLa cells lysate 1200ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:109973(DNTTIP1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
DNTTIP1, also named as Deoxynucleotidyltransferase terminal-interacting protein 1, is a 329 amino acid protein ,which contains a Nuclear localization signal and localizes in the nuclear. DNTTIP1 also acts as a transcriptional regulator, which binding to the consensus sequence 5'-GNTGCATG-3' following an AT-tract. Associates with RAB20 promoter and positively regulates its transcription.
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