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Product Name
DNASE2 antibody
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Description
DNASE2 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 32 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Acid DNase antibody; Deoxyribonuclease 2 alpha antibody; Deoxyribonuclease II alpha antibody; DNase II alpha antibody; DNASE2 antibody; DNASE2A antibody; DNL antibody; DNL2 antibody; Lysosomal DNase II antibody; R31240_2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DNASE2 recombinant protein (Accession Number: NM_001375). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109960(DNASE2 antibody) at dilution of 1:300
IP Result of anti-DNASE2 (IP:Catalog No:109960, 4ug; Detection:Catalog No:109960 1:300) with HeLa cells lysate 3200ug.
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Background
DNASE2, or 'acid DNase,' hydrolyzes DNA under acidic conditions and is one of 2 distinct mammalian DNases. It hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. It has a key role in the degradation of nuclear DNA in cellular apoptosis during development [PMID:18812394]. Besieds, it is necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells. It has been demonstrated to be responsible forDNA degradation in apoptotic cells engulfed by macrophages and exogenous DNA on the skin surface [PMID:23019102].
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