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Product Name
DNA2 antibody
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Description
DNA2 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human cervical cancer tissue. Positive WB detected in mouse thymus tissue. Observed molecular weight by Western-blot: 127 kDa
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
DNA2 antibody; DNA2L antibody; FLJ10063 antibody; KIAA0083 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001080449). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse thymus tissue were subjected to SDS PAGE followed by western blot with Catalog No:110079(DNA2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:110079(DNA2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:110079(DNA2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:110079(DNA2 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
DNA2(DNA replication ATP-dependent helicase/nuclease DNA2) is also named as DNA2L, KIAA0083 and belongs to the DNA2/NAM7 helicase family. This protein includes 2 domains: DNA replication nuclease and DNA replication ATP-dependent helicase. It is a conserved helicase/nuclease involved in the maintenance of mitochondrial and nuclear DNA stability and it can interact with DNA polymerase gamma and stimulating its activity. It can also process intermediate 5' flap structures occuring in DNA replication long-patch base excision repair in mitochondria. It has 4 isoforms produced by alternative splicing. This antibody is specific to DNA2.
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References
- Tsai FL, Kai M. The checkpoint clamp protein Rad9 facilitates DNA-end resection and prevents alternative non-homologous end joining. Cell cycle (Georgetown, Tex.). 13(21):3460-4. 2014.
- Hervouet E, Claude-Taupin A, Gauthier T. The autophagy GABARAPL1 gene is epigenetically regulated in breast cancer models. BMC cancer. 15:729. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"