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Product Name
DMKN antibody
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Description
DMKN Rabbit Polyclonal antibody. Positive IHC detected in human skin tissue, human skin cancer tissue. Positive WB detected in mouse ovary tissue, mouse lung tissue. Positive IP detected in mouse lung tissue. Observed molecular weight by Western-blot: 48-55kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
dermokine antibody; DMKN antibody; FLJ79374 antibody; FLJ79374 antibody; UNQ729 antibody; ZD52F10 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DMKN recombinant protein (Accession Number: XM_017027477). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse ovary tissue were subjected to SDS PAGE followed by western blot with Catalog No:110004(DMKN antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human skin tissue slide using Catalog No:110004(DMKN Antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-DMKN (IP:Catalog No:110004, 4ug; Detection:Catalog No:110004 1:500) with mouse lung tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human skin tissue slide using Catalog No:110004(DMKN Antibody) at dilution of 1:50 (under 10x lens)
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Background
DMKN, also named as epidermis-specific secreted protein SK30/SK89, acts as a soluble regulator of keratinocyte differentiation. DMKN has 16 isoforms with MW 9-20 kDa, 35-38 kDa and 42-48 kDa.
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References
- Tholen S, Biniossek ML, Gansz M. Deletion of cysteine cathepsins B or L yields differential impacts on murine skin proteome and degradome. Molecular & cellular proteomics : MCP. 12(3):611-25. 2013.
- Schlage P, Kockmann T, Sabino F, Kizhakkedathu JN, Auf dem Keller U. Matrix Metalloproteinase 10 Degradomics in Keratinocytes and Epidermal Tissue Identifies Bioactive Substrates With Pleiotropic Functions. Molecular & cellular proteomics : MCP. 14(12):3234-46. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"