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Product Name
DMAP1 antibody
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Description
DMAP1 Rabbit Polyclonal antibody. Positive IP detected in COLO 320 cells. Positive WB detected in NIH/3T3 cells, COLO 320 cells, HeLa cells. Positive IF detected in HEK-293 cells. Observed molecular weight by Western-blot: 50-60 kDa
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Tested applications
ELISA, IP, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp686L09142 antibody; DMAP1 antibody; DNMAP1 antibody; DNMT1 assOCiated protein 1 antibody; DNMTAP1 antibody; EAF2 antibody; FLJ11543 antibody; KIAA1425 antibody; SWC4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DMAP1 recombinant protein (Accession Number: NM_001034023). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IF: 1:20-1:200
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:110001(DMAP1 Antibody) at dilution of 1:1000
IP Result of anti-DMAP1 (IP:Catalog No:110001, 3ug; Detection:Catalog No:110001 1:300) with COLO 320 cells lysate 2500ug.
Immunofluorescent analysis of HEK-293 cells using Catalog No:110001(DMAP1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
DMAP1 (DNA methyltransferase 1-associated protein 1) a component of the NuA4 histone acetyltransferase complex, was originally identified as an interacting molecule with DNA methyltransferase 1 (DNMT1) which co-localizes with PCNA at DNA replication foci. DMAP1 is required for DNA repair and acts against genomic instability. DMAP1 is widely implicated in transcription repression and activation via its interaction with multiple targets including histone deacetylase2 (HDAC2). The antibody reacts specifically with DMAP1 protein.
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References
- Fazzio TG, Huff JT, Panning B. An RNAi screen of chromatin proteins identifies Tip60-p400 as a regulator of embryonic stem cell identity. Cell. 134(1):162-74. 2008.
- Chen PB, Chen HV, Acharya D, Rando OJ, Fazzio TG. R loops regulate promoter-proximal chromatin architecture and cellular differentiation. Nature structural & molecular biology. 22(12):999-1007. 2015.
- Chen PB, Hung JH, Hickman TL. Hdac6 regulates Tip60-p400 function in stem cells. eLife. 2:e01557. 2013.
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