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Product Name
DISC1-long-specific antibody
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Description
DISC1-long-specific Rabbit Polyclonal antibody. Positive WB detected in SH-SY5Y cells, human kidney tissue, mouse brain tissue, mouse kidney tissue. Positive IP detected in mouse brain tissue. Positive IHC detected in human brain tissue. Positive IF detected in MDCK cells. Observed molecular weight by Western-blot: 60kDa, 62 kDa,75- 85 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
C1orf136 antibody; DISC1 antibody; DISC1 long antibody; disrupted in schizophrenia 1 antibody; FLJ13381 antibody; FLJ21640 antibody; FLJ25311 antibody; FLJ41105 antibody; KIAA0457 antibody; SCZD9 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_018662). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:109978(DISC1-long-specific antibody) at dilution of 1:200
Immunohistochemical of paraffin-embedded human brain using Catalog No:109978(DISC1-long-specific antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:109978(DISC1-long-specific antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-DISC1-long-specific (IP:Catalog No:109978, 4ug; Detection:Catalog No:109978 1:300) with mouse brain tissue lysate 2560ug.
Immunofluorescent analysis of MDCK cells using Catalog No:109978(DISC1-long-specific Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
DISC1, also named as KIAA0457, is identified as the sole gene whose ORF is truncated and cosegregates with major mental illnesses in a Scottish family. DISC1 may be implicated in psychiatric conditions in other populations than the unique Scottish family. It is involved in the regulation of multiple aspects of embryonic and adult neurogenesis. DISC1 is required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus. It plays a role, together with PCNT, in the microtubule network formation. In WB, DISC1 has some MW such as 34kd(PMID:18620078 );75-85kd and 95-100kd(PMID:15657124). DISC1 isoforms have distinct developmental patterns of expression. This antibody is against C-terminal of DISC1, binding full length protein specifically.
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