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Product Name
DIO1 antibody
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Description
DIO1 Rabbit Polyclonal antibody. Positive WB detected in A549 cells, HeLa cells, mouse lung tissue, mouse ovary tissue, rat lung tissue, U-937 cells. Positive IP detected in A549 cells. Positive IF detected in A549 cells. Positive IHC detected in human ovary tumor tissue, human kidney tissue, human liver tissue. Observed molecular weight by Western-blot: 13 kDa, 29 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
5DI antibody; DIO1 antibody; DIOI antibody; ITDI1 antibody; TXDI1 antibody; Type 1 DI antibody; Type I 5 deiodinase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DIO1 recombinant protein (Accession Number: NM_001039716). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:109943(DIO1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:109943(DIO1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:109943(DIO1 antibody) at dilution of 1:50 (under 40x lens)
mouse lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:109943(DIO1 Antibody) at dilution of 1:1000
IP Result of anti-DIO1 (IP:Catalog No:109943, 4ug; Detection:Catalog No:109943 1:1000) with A549 cells lysate 1200ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using Catalog No:109943(DIO1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
DIO1(Type I iodothyronine deiodinase) is also named as ITDI1, TXDI1,5DI,DIOI and belongs to the iodothyronine deiodinase family.It is a membrane selenoprotein that catalyzes the deiodination of L-thyroxine to the biologically active thyroid hormone 3,3′,5-triiodothyronine.DIO1 is located in liver, kidney, and thyroid,which has both ORD and IRD activities(PMID: 9389495). It has 9 isoforms produced by alternative splicing with the MW of 29 kDa, 21 kDa, 13 kDa, 23 kDa, 19 kDa, 4 kDa, 13 kDa, 9 kDa and 10 kDa.
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References
- Srivastava J, Robertson CL, Gredler R. Astrocyte Elevated Gene-1 (AEG-1) Contributes to Non-thyroidal Illness Syndrome (NTIS) Associated with Hepatocellular Carcinoma (HCC). The Journal of biological chemistry. 290(25):15549-58. 2015.
- De Andrade PB, Neff LA, Strosova MK. Caloric restriction induces energy-sparing alterations in skeletal muscle contraction, fiber composition and local thyroid hormone metabolism that persist during catch-up fat upon refeeding. Frontiers in physiology. 6:254. 2015.
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