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Product Name
DIAPH3 antibody
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Description
DIAPH3 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, DU 145 cells, HEK-293 cells. Positive IF detected in HeLa cells. Positive IHC detected in human cervical cancer tissue, human prostate cancer tissue. Observed molecular weight by Western-blot: 125-137kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
diap3 antibody; DIAPH3 antibody; Diaphanous related formin 3 antibody; DKFZp434C0931 antibody; DKFZp686A13178 antibody; DRF3 antibody; FLJ34705 antibody; mDia2 antibody; Protein diaphanous homolog 3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DIAPH3 recombinant protein (Accession Number: NM_030932). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109938(DIAPH3 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:109938(DIAPH3 Antibody) at dilution of 1:50
Immunofluorescent analysis of HeLa cells using Catalog No:109938(DIAPH3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
DIAPH3 is also refered as diaphanous-related formin-3 which binds to GTP-bound form of Rho and to profilin, acts in a Rho-dependent manner to recruit profilin to the membrane. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. Seven isoforms of DIAPH3 exist due to the alternative splicing. Recently it has been reported that dimerization and actin assembly activity of DIAPH3 isoforms 1 and 7 are essential for induction of specific cell protrusions. It may propose that isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility.
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References
- Stastna J, Pan X, Wang H. Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation. Cell research. 22(4):728-45. 2012.
- Hetheridge C, Scott AN, Swain RK. The formin FMNL3 is a cytoskeletal regulator of angiogenesis. Journal of cell science. 125(Pt 6):1420-8. 2012.
- Pettee KM, Dvorak KM, Nestor-Kalinoski AL, Eisenmann KM. An mDia2/ROCK signaling axis regulates invasive egress from epithelial ovarian cancer spheroids. PloS one. 9(2):e90371. 2014.
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