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DFF45/DFFA antibody

Cat.#: 109901

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Product Information

  • Product Name
    DFF45/DFFA antibody
  • Documents
  • Description
    DFF45/DFFA Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, HeLa cells. Positive IHC detected in human prostate cancer tissue. Positive IF detected in HEK-293 cells. Observed molecular weight by Western-blot: 45kd
  • Tested applications
    ELISA, WB, IHC, IP, IF
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    DFF 45 antibody; DFF1 antibody; DFF45 antibody; DFF45/DFFA antibody; DFFA antibody; ICAD antibody; Inhibitor of CAD antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of DFF45/DFFA recombinant protein (Accession Number: NM_004401). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109901(DFFA,DFF45 antibody) at dilution of 1:1000

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109901(DFFA,DFF45 antibody) at dilution of 1:1000

    IP Result of anti-DFFA,DFF45 (IP:Catalog No:109901, 3ug; Detection:Catalog No:109901 1:1000) with HEK-293 cells lysate 2500ug.

    IP Result of anti-DFFA,DFF45 (IP:Catalog No:109901, 3ug; Detection:Catalog No:109901 1:1000) with HEK-293 cells lysate 2500ug.

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:109901(DFFA,DFF45 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:109901(DFFA,DFF45 antibody) at dilution of 1:100 (under 10x lens)

    Immunofluorescent analysis of HEK-293 cells using Catalog No:109901(DFFA,DFF45 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HEK-293 cells using Catalog No:109901(DFFA,DFF45 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. DFFA appears to be a 45-kDa protein despite the predicated MW of 37 kDa.
  • References
    • Zhang J, Guo H, Qian G. MiR-145, a new regulator of the DNA fragmentation factor-45 (DFF45)-mediated apoptotic network. Molecular cancer. 9:211. 2010.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"