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Product Name
Dermatopontin antibody
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Description
Dermatopontin Rabbit Polyclonal antibody. Positive IHC detected in human pancreas cancer tissue, human heart tissue, human pancreas tissue, human testis tissue, rat testis tissue. Positive WB detected in mouse skin tissue, human skeletal muscle tissue, rat skin tissue. Observed molecular weight by Western-blot: 22 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Dermatopontin antibody; DPT antibody; TRAMP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Dermatopontin recombinant protein (Accession Number: NM_001937). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse skin tissue were subjected to SDS PAGE followed by western blot with Catalog No:109894(DPT Antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:109894(DPT antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:109894(DPT antibody) at dilution of 1:100 (under 40x lens)
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Background
DPT (dermatopontin) is a 22 kDa extracellular matrix protein belonging to the dermatopontin family. Skin appears to be the richest source of dermatopontin, comprising about 15 mg/kg of the wet weight. Expression of dermatopontin is not limited to connective tissue, as investigations show that dermatopontin mRNA is expressed in cultured fibroblasts, muscle, heart, pancreas, and lung. Dermatopontin comprises a considerable proportion of the noncollagenous extracellular matrix proteins. It is involved in promotion of cellular adhesion, extracellular matrix assembly, wound healing, and positive modification of the growth inhibition activity of TGF-beta1.
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References
- Hinsch N, Frank M, Döring C, Vorländer C, Hansmann ML. QPRT: a potential marker for follicular thyroid carcinoma including minimal invasive variant; a gene expression, RNA and immunohistochemical study. BMC cancer. 9:93. 2009.
- Li X, Feng P, Ou J. Dermatopontin is expressed in human liver and is downregulated in hepatocellular carcinoma. Biochemistry. Biokhimii︠a︡. 74(9):979-85. 2009.
- Yamatoji M, Kasamatsu A, Kouzu Y. Dermatopontin: a potential predictor for metastasis of human oral cancer. International journal of cancer. 130(12):2903-11. 2012.
- Liu X, Meng L, Shi Q. Dermatopontin promotes adhesion, spreading and migration of cardiac fibroblasts in vitro. Matrix biology : journal of the International Society for Matrix Biology. 32(1):23-31. 2013.
- Fu Y, Feng MX, Yu J. DNA methylation-mediated silencing of matricellular protein dermatopontin promotes hepatocellular carcinoma metastasis by α3β1 integrin-Rho GTPase signaling. Oncotarget. 5(16):6701-15. 2014.
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