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Product Name
DEPDC6 antibody
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Description
DEPDC6 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A2780 cells, COLO 320 cells, HEK-293 cells, MCF7 cells. Positive IHC detected in human liver cancer. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 40-45kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DEPDC6 antibody; FLJ12341 antibody; DKFZp564B1778 antibody; Deptor antibody; DEP.6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_022783). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109892(DEPDC6 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:109892(DEPDC6 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:109892(DEPDC6 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:109892(DEPDC6 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
DEPDC6, also named as DEPTOR, was identified as a novel mTOR-interacting protein whose over-expression in certain cell types represents a new mechanism for activating PI3K/Akt signaling and promoting cell survival. It negative regulator of the mTORC1 and mTORC2 signaling pathways. DEPDC6 inhibits the kinase activity of both complexes. The antibody is specific to DEPDC6.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"